Table 1 Primer sequences and optimal conditions used in PCR analysis
Gene | Sense primer sequence (5′–3′) | Antisense primer sequence (5′–3′) | Amplification conditions | Amplification cycles, n |
|---|---|---|---|---|
SAA3 | AGC CTT CCA TTG CCA TCA TTC TT | AGT ATC TTT TAG GCA GGC CAG CA | 94°C 1min; 58°C 1min; 74°C 1min | 25 |
IP10 | GTGTTGACATCATTGCCACG | GCTTACAGTACAGAGCTAGG | 95°C 30 sec; 60°C 30 sec; 45°C 45 sec | 30 |
CCL-2 | CCCCACTCACCTGCTGCTACT | CACTGTCACACTGGTCACTCC | 95°C 50 sec; 64°C 50 sec; 72°C 1 min | 36 |
CCL-3 | ACTGCCCTTGCTGTTCTTCTCT | AGGCATTCAGTTCCAGGTCAGTGA | 95°C 30 sec; 61°C 30 sec; 72°C 30 sec | 33 |
CCL-4 | CCCTCTCTCTCCTCTTGCTCGT | TTCAACTCCAAGTCACTCATGTACTCA | 94°C 30 sec; 55°C 30 sec; 72°C 1 min | 32 |
CXCL-2 | GCTGTCAATGCCTGAAGACC | TAGTTCCCAACTCACCCTCTC | 95°C 50 sec; 65°C 50 sec; 72°C 1 min | 36 |
IL-1β | AAGGAGAACCAAGCAACGAC | GAGATTGAGCTGTCTGCTCA | 95°C 50 sec; 63°C 50 sec; 72°C 1 min | 34 |
CCR-2 | CACGAAGTATCCAAGAGCTT | CATGCTCTTCAGCTTTTTAC | 94°C 30 sec; 58°C 45 sec; 72°C 70 sec | 35 |
CCR-5 | TTCCCTGTCATCGCTTGCTCT | CGGATGGAGATGCCGATTTT | 94°C 1 min; 60°C 1 min; 72°C 2 min | 40 |
IL-1ra | GACCCTGCAAGATGCAAGCC | CAGGACGGTCAGCCTCTAGT | 95°C 20 sec; 51°C 20 sec; 72°C 20 sec | 36 |
18S | GTA ACC CGT TGA ACC CCA TT | CCA TCC AAT CGG TAG TAG CG | 95°C 30 sec; 59°C 30 sec; 72°C 30 sec | 24 |