Figure 1
From: Interferon γ-Induced Human Guanylate Binding Protein 1 Inhibits Mammary Tumor Growth in Mice
Characterization of TS/A mammary carcinoma cells inducibly expressing hGBP-1. (A) Western Blot analysis of TS/A cell clones 13–18 harboring the Tet on/off regulatory system and the vector pUHD10.3-GBP-Hyg, mediating tightly regulated, inducible expression of hGBP-1 in response to doxycycline (+ dox). As a control for analysis of equal amounts of protein, a Coomassie-stained SDS-polyacrylamide gel with the respective samples is depicted (lower panel). (B) Analysis of proliferation characteristics of pUHD10.3-GBP-transfected cells (cell population, gray bars; cell clone, white bars) in comparison to pUHD10.3-Hyg-transfected cells (black bars). Cell numbers (mean values ± SD) from three independent experiments are shown in % relative to nontreated (− dox) samples. (C) Soft agar assay. Nontransfected TS/A cells and TS/A cells transfected with pUHD10.3-Hyg or the hGBP-1-encoding vector (pUHD10.3-GBP) were analyzed for their ability to form colonies in soft agar. Expression of hGBP-1 was induced by addition of 1 (gray bars) or 2.5 (black bars) µg/mL doxycycline and the number of colonies (mean values ± SD) obtained by counting 10 different optical fields compared with nontreated samples (white bars).