Figure 3
From: Interferon γ-Induced Human Guanylate Binding Protein 1 Inhibits Mammary Tumor Growth in Mice
Evaluation of effects of hGBP-1 expression on tumor cell proliferation. (A) Immunohistochemistry. Consecutive sections of hGBP-1-expressing (TS/A-GBP + dox) and nonexpressing (TS/A-Hyg + dox) tumors were stained for expression of hGBP-1 (α-hGBP-1, brown cytoplasmic staining) and murine Ki-67 (α-mKi-67, red nuclear staining), a cellular proliferation marker. Using the Zeiss AxioVision analysis software, sections were divided into different areas correlating with a high level (a, b, d, e, g, h, i) or low level (c, f) hGBP-1 expression. (B) The number of Ki-67-positive TS/A cells was calculated and expressed as mean values per 100.000 µm2. Statistical analysis revealed significant differences (P = 0.017) in the number of Ki-67-positive cells (mean values ± SD) in hGBP-1-expressing (total GBP+, n = 11) compared with nonexpressing tumors (total GBP−, n = 10). Highly significant differences (P < 0.0001, one-way ANOVA) were observed when Ki-67-positive cell numbers were correlated with hGBP-1 expression levels.