Figure 4

Reduced expression of SPRR3 does not affect cell proliferation and cell death in colorectal cancer cells. The SW480 cells were transfected with control scrambled siRNA (Scram) or SPRR3 siRNA (siSPRR3). Reduced SPRR3 expression was confirmed by Western blot analysis with anti-SPRR3 antibody. Two days after siRNA transfection, cells were exposed to 50 ug/mL 5 FU with 10 ug/mL Leucovorin (FL), 30 ug/mL Oxaliplatin (Oxal) or 30 ug/mL Irinotecan (Irino) for 24 h and then cell viability was determined by MTT assay. The values are mean ± SEM (n > 3 times).