Figure 1

Cisplatin-induced apoptosis in BUMPT cells. (A) BUMPT cells were untreated or treated with 40 µmol/L cisplatin for 20 h. Cell morphology was recorded by phase-contrast microscopy (10×). (B) BUMPT cells were treated with 40 µmol/L cisplatin for the indicated time. Whole-cell lysates were collected for immunoblot analysis of Phospho-p53 (Ser-15), Puma-α, p21, and β-actin (protein loading and transferring control). (C) BUMPT cells were untreated or treated with 40 µmol/L cisplatin for 24 h in the absence or presence of 20 µmol/L pifithrin-α. Percentage of apoptosis was determined by counting the cells with typical apoptotic morphology. (D) BUMPT cells were treated with 40 µmol/L cisplatin for 20 h in the absence or presence of 20 µmol/L pifithrin-α. Cell lysates were collected to measure caspase activity by an enzymatic assay as described in Methods. Data in C, D: mean ± SD, n = 3; *, significantly different from the cisplatin group.