Figure 3

MicroRNA-34a induction during cisplatin treatment of C57BL/6 mice. Male C57BL/6 mice (age 8–10 wks) were injected with 30 mg/kg body weight cisplatin. Kidneys were collected at d 1, 2 and 3 after cisplatin treatment for the isolation of total RNA. (A) Northen blot analysis of miR-34a expression in renal tissues. Total RNA (10 µg) from renal tissues was subjected to Northern blot analysis using a specific miR-34a probe. 5S RNA was recorded as a sample loading control. Left panel, Northern blots; right panel, densitometry analysis. For densitometry, the miR-34a signal of each condition was normalized with its 5S-RNA signal and then compared with that of the control (0 cisplatin), which was arbitrarily set as one. (B) Real-time PCR analysis of miR-34a. Total RNA was isolated from sham control mice and mice with 3 d of cisplatin treatment for real-time PCR using miR-34a-specific primers. snoRNA-202 was also analyzed in all samples as an endogenous control for normalization. Data: mean ± SD, n = 3; *, significantly different from the control group.