Figure 5

Blockade of miR-34a increased cisplatin-induced apoptosis in BUMPT cells. BUMPT cells were transfected with 100 nmol/L LNA-modified antisense oligonucleotide of miR-34a (anti-miR-34a) or LNA-modified oligonucleotide of scrambled sequence (scrambled). The cells were then left untreated or treated for 20 h with 40 µmol/L cisplatin. (A) Effects of anti-miR-34a on miR-34a induction during cisplatin treatment. Total RNA was isolated for real-time PCR analysis of miR-34a using specific primers. (B) Effects of anti-miR-34a on cisplatin-induced apoptosis. Percentage of apoptotic cells was determined by morphological methods. (C) Effects of anti-miR-34a on cisplatin-induced caspase activation. Cell lysate was collected for an enzymatic assay of caspase activity. (D) Effects of anti-miR-34a on cell survival following cisplatin treatment. After cisplatin treatment for 24 h, the cells were incubated in fresh medium for 24 h of recovery. Whole cell lysate was then collected to determine the recovered protein to indicate cell survival. Data: mean ± SD, n = 3; *, significantly different from control and scrambled groups.