Figure 6

Effect of H₂S donor NaHS administration on MPO activity in lung (A) and liver (B) from mice subjected to burn injury. Mice subjected to burn injury were randomly given NaHS (10 mg/kg i.p.) or saline at the same time of burn injury. Eight hours after burn injury, MPO activity in lung and liver was measured as described in Materials and Methods. Results shown are mean ± SEM (n = 9 animals/group). ^##P <0.01 when NaHS-treated mice were compared with saline-treated mice. (C–F) Morphological changes in lung and liver from mice subjected to burn injury with NaHS administration in hematoxylin and eosin-stained sections. (C) Lung from mice subjected to burn injury with saline simultaneously; (D) lung from mice subjected to burn injury with NaHS simultaneously; leukocyte infiltration and alveolar wall thickness (arrow) can be easily detected. (E) Liver from mice subjected to burn injury with saline simultaneously; (F) liver from mice subjected to burn injury with NaHS simultaneously. The scale bar is 2 µm.