Skip to main content
Figure 5 | Molecular Medicine

Figure 5

From: Peroxynitrite-induced Nitration of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Promotes Their Binding in Diabetic Angiopathy

Figure 5

Content and nitration level of coimmunoprecipitated COX-2 and iNOS in the rat aorta and renal cortex. The coimmunoprecipitated COX-2 or iNOS and their nitration level in the aorta (A) and renal cortex (B) were detected by coimmunoprecipitation and Western blotting. The ratio of the coimmunoprecipitated COX-2 or iNOS to its own total protein content was calculated to eliminate the effect of total protein content in the aorta (C) and renal cortex (D), respectively. The ratio of NT content to the content of coimmunoprecipitated COX-2 or iNOS was calculated to show their nitration level in the aorta and renal cortex, respectively (E and F). Data were means ± SD (n = 3 in each group, repeated twice). *P < 0.05 versus the diabetes group.

Back to article page

Molecular Medicine

ISSN: 1528-3658