Figure 6

Characterization of the degree of reduction of IR expression in IR KO KLE cells (A) and of IGF-1R expression in IGF-1R KO KLE cells (B) by qRT-PCR. IR KO and IGF-1R KO lines represent the melting curves of IR (IR-A and IR-B isoforms) and IGF-1R in IR KO KLE and IGF-1R KO KLE cells, respectively, compared with the lines of IR and IGF-1R in control KLE cells. The amplification curves and Cts (D, E) as well as normalized expression (ddCt) charts (F, G) are also shown. The degree of reduction of IR and IGF-1R expression was from 60% up to 80% in this cell line. Normalization in all the cases was carried out by β-actin (C).