Insulinlike Growth Factor-1Ec (MGF) Expression in Eutopic and Ectopic Endometrium: Characterization of the MGF E-Peptide Actions In Vitro

Table 1 The effects of 48 h of treatment with mature IGF-1, insulin and synthetic MGF E-peptide on KLE cell proliferation, as assessed by Trypan blue exclusion assays (cell number × 10⁴).

IGF-1 (50 ng/mL) in untransfected KLE cells	MGF (50 ng/mL) in untransfected KLE cells	IGF-1 (50 ng/mL) in IGF1R siRNA KLE cells	MGF (50 ng/mL) in IGF1R siRNA KLE cells	Control siRNA-transfected KLE cells
112.5 ± 8.66 a	103.33 ± 5.20 b	76.25 ± 5.30	95.83 ± 3.81 c	73.75 ± 5.30
Insulin (30 ng/mL) in untransfected KLE cells	MGF (50 ng/mL) in untransfected KLE cells	Insulin (30 ng/mL) in IR siRNA KLE cells	MGF (50 ng/mL) in IR siRNA KLE cells	Control siRNA-transfected KLE cells
32.9 ± 5.49 b	34.37 ± 5.15 a	12.5 ± 2.5	28.87 ± 4.73 b	11.25 ± 2.5

The mitogenic activity of the IGF-1 and insulin was blocked in IGF-1R siRNA KLE cells and in the IR siRNA KLE cells, respectively, whereas MGF E-peptide mitogenic actions were not affected in IGF-1R siRNA KLE cells and in the IR siRNA KLE cells. These data suggested that MGF actions are possibly mediated via an IGF-1R-independent, IR-independent and hybrid IGF-1R/IR-independent mechanism in KLE endometrial-like cells. Significantly different from control-siRNA transfected KLE cells: ^aP< 0.001; ^bP< 0.01; ^cP< 0.05.

ISSN: 1528-3658