Figure 5
From: mTOR Regulates the Invasive Properties of Synovial Fibroblasts in Rheumatoid Arthritis
Rapamycin significantly reduced RA FLS invasion and FAK phosphorylation. (A) Western blot demonstrating reduced levels of phospho-FAK in rapamycin-treated (R) DA FLS, compared with DMSO-treated FLS (D). (B) Densitometry analyses showing phospho-FAK/total FAK in RA cells stimulated for 30 min. (C) Rapamycin (100 nmol/L) also significantly reduced RA FLS median invasion by 82% compared with DMSO alone (n = 8; *P < 0.001, Mann-Whitney test; 25%–75% confidence intervals = 5.42–23.78).