Figure 4

Effects of human AM/AMBP-1 on neuron apoptosis after stroke. Apoptotic cells were identified by TUNEL assay (green fluorescence labeling). (A) Photomicrography of a coronal section at the bregma level from a sham-operated rat. (B) Photomicrography of the penumbra area at a coronal section at the bregma level from a stroke rat at 24 h after permanent MCAO treated with human albumin (Vehicle). (C) Photomicrography of the penumbra area at a coronal section at the bregma level from a stroke rat at 24 h after MCAO treated with human AM/AMBP-1 (AM/AMBP-1). (D-F) Photomicrography of brain sections from corresponding slide (A-C) propidium iodide staining (showing nuclei). (G) Quantitative determination of the TUNEL-positive cells in sham-operated animals (Sham) and stroke animals treated with human albumin (Vehicle), or human AM/AMBP-1 (AM/AMBP-1) at 24 h after permanent MCAO. Data are presented as means ± SE (n = 6/group) and compared by one-way ANOVA and Student-Newman-Keul test: *P < 0.05 versus sham group; ^#P < 0.05 versus vehicle group. Original magnification 200x. Scale bar = 50 µm.