Figure 2

ITF2357 protects from cytokine-induced islet injury in vitro. Primary mouse islets were incubated for 1 h with increasing concentrations of ITF2357 or vehicle (water) and then IL-1 β (10 ng/mL) plus IFNγ (25 ng/mL) were added. The data are derived from three independent experiments. Control (CT) islets were cultured without added cytokines. After 48 h, cell viability, supernatant nitric oxide and chemokine levels were measured and apoptosis rates were determined. Data from cytokine-stimulated islet cultures were set at 100% and the percent change was calculated for each concentration of ITF2357. (A) Mean ± SEM percent change in nitric oxide. (B) Cell viability, as determined by MTT reaction, depicted as fold-change from CT. (C and D) Mean ± SEM percent change in MIP-1α and MIP-2. *P< 0.05, **P< 0.01, ***P< 0.005 compared with stimulated islets in the presence of vehicle. (E) Apoptosis, as determined by sub-G1 population evaluation of PI-stained islet single-cell suspension. ITF2357 (100 nmol/L) in the presence of IL-12 (2 ng) plus IL-18 (20 ng)/mL.