HIV-pulsed DCs impair T-cell proliferation. Mature DCs were pulsed with HIV (750 ng/mL) for 24 h, washed and cocultured with CFSE-labeled naïve bulk T cells at a ratio of 1:10 (DC:T cells). Assays were restimulated after 7 d and T-cell proliferation was measured after 24 h (d 8) by flow cytometry. (A) Mock- and HIV-pulsed DCs cocultured with naïve T cells (a representative of one experiment). (B) Ten combined experiments normalized together (P < 0.001).