Figure 4
From: Retroviral Insertional Mutagenesis Can Contribute to Immortalization of Mature T Lymphocytes
The immortalized T-cell population is monoclonal for the T-cell receptor (Vβ13). (A) Compared to a WT population, where all TCR Vβ variants were found (data not shown), only Vβ13 could be PCR amplified in immortalized cells from cDNA; M, Marker; H2O, negative control. (B) This result was further verified by FACS-mediated detection of Vβ13 on the majority of cells, while all other Vβ antibodies were nonreactive.