Figure 5
From: Retroviral Insertional Mutagenesis Can Contribute to Immortalization of Mature T Lymphocytes
Retroviral integration site analysis of the immortalized T-cell population. (A) Compared to a freshly stimulated and transduced T-cell population (1) the immortalized cells (2) showed an oligo- to monoclonal integration pattern. One of the integrations of the immortalized population was mapped near the genes of IL2RA and IL15RA (*). Arrow marks the internal control band. M, Marker. (B) Scheme of retroviral integration site (RIS) mapped upstream of the IL2RA and the long isoform of the IL15RA loci. Black blocks indicate IL2RA and IL15RA exons. The arrowhead represents RIS in reverse (RV) vector orientation. Black arrows show transcription start sites (TSS).