Figure 6

Expression profile of immortalized T cells compared with fresh mature and immature T cells. RNA was purified from freshly isolated T lymphocytes from spleen and lymph nodes and thymocytes from 8-wk-old WT donors and the immortalized T-cell clone. Miltenyi Biotech performed Microarray ratio experiments commercially. Immortalized T cells were compared with freshly isolated mature (A) and immature T cells (B). The signal intensities of each feature represented by a spot are shown in a double logarithmic scale as a scatter blot. x Axis: mature T cells (A) or thymocytes (B) log signal intensity; y axis: immortalized T-cell clone (A,B) log signal intensity. Red diagonal lines define the areas of 2-fold differential signal intensities. Blue crosses: unchanged genes. Red crosses: significantly up-regulated genes (P value < 0.01). Green crosses: significantly downregulated genes (P value < 0.01). Grey cross in legend: summary of significantly up- and downregulated signatures. No similarities to one or the other cell type were observed for the investigated immortalized cells. As expected, the transduced LMO2 gene and the retroviral integration site (RIS) flanking genes IL2RA and IL15RA showed increased expression in the immortalized cells relative to fresh mature and immature WT T cells.