Figure 3

Tissue specificity of GNMT disruption on folate status. GNMT knockout significantly reduced folate concentrations (A) in plasma; (B) in GNMT expressing tissue liver; (C) but not in non-GNMT-expressing tissue spleen. GNMT wild-type (GNMT^wt), heterozygous (GNMT^het), homozygous (GNMT^nul) mice were bred from crossing heterozygous (GNMT^het) parents that received amino acid defined diet with 2 mg/kg folic acid and 0.1 % sulfathiazole. The offspring mice were fed the same diet after weaning.