Figure 2
Ang II-induced migration of keloid fibroblasts (A) Ang II induces keloid fibroblast migration in a dose-dependent manner. Ang II (10−5 to 10−8 mol/L) was added to the medium, and keloid fibroblast migration was assessed by a Boyden chamber assay. (B) Ang II-induced migration of keloid fibroblasts is mediated by the AT,. EMD66684 and losartan (AT1, antagonists) or PD,233,9 (AT2 antagonist) was added to the medium at a concentration of 10−5 mol/L and keloid fibroblast migration assessed in response to 10−6 mol/L Ang II. EMD66684 and losartan, but not PD123319, inhibited Ang II-induced keloid fibroblast migration. (C) Effects of the nonmuscle myosin II inhibitor blebbistatin on Ang II-induced keloid fi-broblast migration. Blebbistatin (0–20 µmol/L) was added to the medium, after which Ang II-induced migration was assessed by a Boyden chamber assay. Blebbistatin inhibition of Ang II-induced keloid fibroblast migration was dose dependent. All data are presented as percentage of migration in the absence of Ang II, mean ± SEM; n = 9; *P ≤ 0.05.