Figure 6
Restoration of ANCA-induced exocytosis with LPC and ionomycin. Primed neutrophils in the presence of cytochalasin B (2.5 × 105) were treated with ANCA IgG (200 µg/mL) or control IgG (200 µg/mL) for 15 min in the presence of LPC (250 nmol/L) and ionomycin (5 nmol/L) alone or together in the same treatment sample. Paired samples of cells were treated with R59022 (18 µmol/L) for 10 min before priming. Supernatants were collected and analyzed for the activity of MPO using a specific substrate. Data have been normalized as a percentage of the unstimulated (unstim), uninhibited control cells. Data are the mean of seven different neutrophil donor responses using a panel of two normal IgG preparations and two ANCA preparations (one MPO-ANCA, one PR3-ANCA). *P < 0.05 by one-way ANOVA with posttest.