Figure 1
From: Effect of Oxygen Levels on the Physiology of Dendritic Cells: Implications for Adoptive Cell Therapy
Endocytic capacity of immature DCs generated at atmosO2 and physO2 conditions. Immature DCs differentiated at atmospheric or physiological oxygen levels were harvested and incubated with 1 mg/mL Dextran-FITC for 30 min at 37°C at atmospheric oxygen levels (A, B) or at physiological oxygen levels (C). Cells were subsequently washed, fixed and analyzed by flow cytometry. (A) Immature DCs exposed to media are shown in the shaded histogram as the control. PhysO2 immature DCs (dotted line) and atmosO2 immature DCs (solid line) were exposed to Dextran-FITC at atmosO2. One representative experiment out of two is shown. (B) The mean fluorescence intensities (MFI) ± SD of four experiments, where DCs were exposed to Dextran-FITC at atmosO2 using DCs from different donors. *Statistically significant difference, P < 0.05, paired Student t test. (C) MFI ± SD of four experiments conducted where DCs were exposed to Dextran-FITC at physO2 levels using DCs from different donors. No significance was observed.