Figure 2
From: Low-Dose Methotrexate Inhibits Methionine S-Adenosyltransferase In Vitro and In Vivo
Folinate or methionine supplementation restored S-adenosylmethionine levels in folate-depleted cells but not in MTX pretreated cells. (A) Effects of folate or methionine supplementation on intracellular S-adenosylmethionine (AdoMet) levels in folate-depleted or MTX pretreated HepG2 cells (P > 0.05). MTX: treated with 50 nmol/L MTX for 72 h, FA def: folate restricted (10 nmol/L) for 144 h. Post-FA sup: supplemented with 100 nmol/L folinate for additional 48 h in MTX pretreated cells, Post-Met sup: supplemented with 500 µmol/L methionine for additional 48h in MTX pretreated cells. (B) Effects of cosupplemented MTX with folinate or methionine on intracellular S-adenosylmethionine (AdoMet) levels in HepG2 cells. Co-FA sup: cosupplemented MTX with 100 nmol/L folinate for 72 h, Co-Met sup: cosupplemented MTX with 500 µmol/L methionine for 72 h. Data are shown as mean ± SE. The values with different letters were significantly different (n = 4 for each treatment).