Figure 3
From: Low-Dose Methotrexate Inhibits Methionine S-Adenosyltransferase In Vitro and In Vivo
Effects of folate restriction on MAT expression and enzyme activity in HepG2 cells. (A) MAT2A mRNA. (B) MAT II protein. (C) MAT enzyme activity (n = 4 for each treatment). Data are shown as mean ± SE. Folate restriction significantly induced MAT 2A mRNA expression by 50% compared with controls (P= 0.005, n = 3 per group). No significant difference was found in MAT II protein expression levels or MAT enzyme activity between folate replete and depleted cells.