Figure 6From: Low-Dose Methotrexate Inhibits Methionine S-Adenosyltransferase In Vitro and In VivoEffects of MTX treatment on MAT activity in cell free protein lysates. Cell free protein lysates from (A) untreated HepG2 cells or (B) healthy mice liver tissue were treated with MTX (from 500 to 2500 µmol/L), and MAT activity was determined as described in Materials and Methods. MTX did not alter MAT activity in cell-free lysates.Back to article page