Figure 7
From: Low-Dose Methotrexate Inhibits Methionine S-Adenosyltransferase In Vitro and In Vivo
MTX treatment suppressed dexamethasone-induced MAT2A gene expression. MTX: cells were treated with 50 nmol/L MTX for 72 h. DEX: treated with 100 nmol/L dexamethasone for 72 h. ACD: treated with 5 µg/mL actinomycin D for 12 h before harvest. DEX + ACD: treated with DEX for 72 h, at 60 h, the 5 µg/mL actinomycin D was added for additional 12 h. DEX + MTX: the cells were cotreated with 50 nmol/L MTX and 100 nmol/L DEX for 72 h. Data are shown as mean ± SE (n = 3). The values with different letters are significantly different (P< 0.05).