Figure 8
From: Low-Dose Methotrexate Inhibits Methionine S-Adenosyltransferase In Vitro and In Vivo
Concurrent folinate supplementation ameliorated MAT2A mRNA reduction by MTX. HepG2 cells received folinate (100 nmol/L, +F), MTX (50 nmol/L, +M), concurrent folinate supplementation with MTX (+M+F), or folinate supplementation after MTX exposure for different time periods. Cells were harvested and MAT2Agene expression levels were determined as described in Materials and Methods. Data are shown as mean ± SE. The values with different letters are significantly different (n = 3).