Figure 1

The expression of H3K27me3 in NPC cell lines and tissues. (A) Upregulated expression of H3K27me3 was observed in 17/18 of NPC tissues compared with nonneoplastic nasopharyngeal mucosal tissues. T, NPC tissue; N, nonneoplastic nasopharyngeal mucosal tissue. (B) The eight NPC cell lines (that is, CNE1, CNE2, C666, HONE1, HNE1, 5–8F, SUNE1 and 6–10B) exhibited relatively higher levels of H3K27me3 expression than that in the immortalized normal human nasopharyngeal epithelial cell line (NP69). (C) High expression of H3K27me3 was shown in a NPC case (case 36), in which more than 75% of carcinoma cells revealed positive staining of H3K27me3 protein in nuclei (100). (D) Another NPC case (case 27) demonstrated low expression of H3K27me3, in which less than 30% of carcinoma cells showed positive staining of H3K27me3 protein in nuclei (100). (E) Normal nasopharyngeal mucosal tissue showed nearly negative expression of H3K27me3 protein, though immunoreactivity also could be seen in some stromal lymphocytes (100). The lower panels indicated the higher magnification (400×) from the area of the box in the upper panels.