Figure 2

ITF2357 ameliorates both murine CIA and rat AA. (A) Murine type II collagen-induced arthritis was induced according to standard procedures. DBA-1 mice were treated orally with vehicle or ITF2357 at 1 and 10 mg/kg/day. Dexamethasone (0.25mg/kg) was used as positive control (n = 10 mice per group). Treatment was started just after the mice were immunized with CII/FCA (prophylactic). Mice were examined visually for arthritis expression. Noted that ITF2357 showed a potent antiinflammatory effect at a dose of 10 mg/kg/day. (B) ITF2357 was administered orally at a dose of 10 mg/kg in a prophylactic regimen (as in panel A) or starting on day 22 after arthritis induction (therapeutic regimen). (C) Rat adjuvant arthritis (AA) treated with ITF2357 (5 and 50 mg/kg). AA was induced using a standard protocol. Rats (n = 8 per group) were treated for 21 d, starting directly after immunization with FCA/MTB. Leflunomide (10 mg/kg/day) was used as positive control. Potent suppression of AA with both ITF2357 (50 mg/kg) and Leflunomide (10 mg/kg). Mann-Whitney U test was used for statistical analysis.