mg/kg | Inhibition (%) of synovial cytokine production after ITF2357 treatment |
|---|
TNFα | IL-1β | IL-1 a | IL-6 | IFNy | IL-12 | MIP-1a | KC |
|---|
1 | −11.8 | 16.7 | 36. 7 | −9.3 | 4.8 | 0.5 | 0.7 | 17.4 |
5 | 17.5 | 30.1 | 35.2 | 14.3 | 42.1 | 23.8 | 17.2 | 32.0 |
10 | 45.3 | 61.7 | 66.3 | 39.3 | 59.1 | 64.2 | 51.9 | 25.8 |
- Joint inflammation was induced by injection of 25 µg SCW fragments into the right knee joint of C57/Bl6 mice 2 h after ITF2357 had been supplied orally (n = 5 mice per group). After 4 h, patellae with surrounding synovial tissue were isolated and cultured for 1 h at RT in RPMI 1640 medium. Cytokines were determined by Luminex bead array system. Values represent the reduction in cytokine production compared with vehicle control. A negative value indicates induction of cytokines.
