Effect of Deptor KD on C2C12 differentiation. (A) Myoblasts were transfected with either control (scramble) shRNA or shRNA targeting Deptor. Cells were plated at the same density and photographed daily (10× objective magnification) to visually record changes in cell proliferation (time to reach confluence) and formation of myotubes. On days 3–4, when the plates were confluent, the media were switched to 2% horse serum (DM = differentiation media) to induce myotube formation. Deptor KD in C2C12 myocytes enhances MHC protein expression. (B) Representative Western blots for Deptor protein, MHC and the muscle-specific transcription factor MyoD in samples treated as in A. β-Tubulin serves as a loading control.