The efficiency of siRNA in knockdown of CD31 and JNK in HUVECs. (A) Western blot image of CD31 expression and ß-actin with or without si-CD31 or low shear stress pretreatment. (B) Relative quantitation of CD31 normalized to control of static group in different groups. (C) Western blot image of t-JNK expression after si-CD31 or low shear stress treatments. (D) Quantitation of relative JNK level normalized to control of static group. Each data point was obtained from triplicate experiments. ▲P < 0.05 versus static control. ●P < 0.05 versus low shear stress control.