Galantamine reduces alanine aminotransferase levels, liver weight and hepatocyte lipid accumulation in mice with high-fat diet-induced obesity. Plasma ALT levels and liver weights were determined in mice fed a low-fat diet and treated with saline (LFD-S), high-fat diet and treated with saline (HFD-S) or high-fat diet and treated with galantamine (HFD-G). Following H&E staining of liver tissue slides, lipid accumulation was determined by microscopic histological evaluation and applying grading criteria as described in Materials and Methods. (A) Plasma ALT levels, (B) liver weight, (C) Representative photomicrographs (×20) of H&E-stained liver section slides in: LFD-S mice; HFD-S mice; and HFD-G mice. (D) Hepatocyte lipid accumulation in the three groups of mice (n = 7 per group with representative average body and liver weights). (#P < 0.05 versus LFD-S, *P < 0.05 versus HFD-S).