Figure 1
Characterization of expression levels of M3-mAChR in α-MHC promoter M3-mAChR transgenic gene (M3Tg) mice. (A) Schematic of the M3-mAChR gene constructed in the α-MHC promoter-M3Tg. The 13-kb transgene construct used to produce the transgenic mice was composed of cDNA (1,836 bp) of M3 from the UMR cDNA Resource Center, subcloned between SaII and HindIII of the α-MHC expression cassette. (B) The mRNA expression of M3-mAChR determined by real-time RT-PCR of total RNA in WT and M3Tg mice. (C) Top, examples of Western blot bands; bottom, expression levels of M3-mAChR. (D) Raw traces of IKM3 recorded from WT and M3Tg mice. (E) I-V relationships of IKM3 currents in WT and M3Tg mice. The average data presented were mean ± SEM; *P < 0.05, *P < 0.01 versus WT.