Figure 5
Alterations of protein and mRNA levels of IK1 and Ito revealed by Western blot analysis and real-time RT-PCR. (A) Top, examples of Western blot bands of Kir2.1; bottom, quantification of Kir2.1 (B) Top, examples of Western blot bands of Kv4.2; bottom, quantification of Kv4.2. (C) Top, examples of Western blot bands of Cav1.2; bottom, quantification of Cav1.2. (D) Effects of transgenic M3-mAChR on expression of KCNJ2 determined by real-time RT-PCR of total RNA from control and myocardial I/R mice. (E) Effects of transgenic M3-mAChR on expression of KCND2 determined by real-time RT-PCR of total RNA from healthy and myocardial I/R mice. (F) Effects of transgenic M3-mAChR on expression of CACNA1C determined by real-time RT-PCR of total RNA from control and myocardial I/R mice. The average data were presented as mean ± SEM. **P < 0.01 versus WT or WT I/R mice.