cAng-1 restored ultrastructural vascular integrity in the IR myocardium. (A) Transmission electron micorsscope (TEM) analysis of IR tissues at 24 h after surgery. (a, b) Sham group. (c–f) IR/Ad-β-gal group. (e, f) Enlarged view of the area indicated as rectangles from (d). Arrows, vessel breakdown; arrowhead, vacuole in endothelium. (g, h) IR/Ad-cAng-1 tissue. Arrows, cardiomyocyte damage. Breakdown of vascular integrity and endothelial/cardiomyocyte damages are frequently observed in the IR myocardium, but cAng-1 treatment prevented such damages. Images are representative ones from each group. RBC, red blood cells. Scale bar = 2 μm. (B) Immunoprecipitation of cardiac protein with anti-VE-cadherin antibody followed by immunoblotting with anti-SHP2 or anti-PTPμ antibody. Binding of SHP2/VE-cadherin or PTPμ/VE-cadherin was notably decreased in the IR myocardium, which was remarkably restored by Ad-cAng-1 treatment (n = 3). WCL, whole cell lysate.