Figure 2

MACs promote vascular repair in the ischaemic retina. (A) Injected MACs labeled with red Qdots did not incorporate into the resident vascular network labeled in green with isolectin. Scale bars: 100 µm. (B) Injected human MACs retained a myeloid phenotype as demonstrated by expression of human-specific CD68 and typical amoeboid morphology. Scale bars: 75 µm. (C) Representative flat mounted retinas injected with vehicle (Control) or MACs, respectively. Isolectin staining in green identifies retinal vasculature, and avascular regions are outlined in yellow. Insets show ischaemic areas in white. (D) Quantification of avascular areas and ratio of avascular/total retinal area shows that injection of MACs into the eye significantly induced vascular repair compared with injection of vehicle injected. *P < 0.05 versus control.