Figure 2

Analysis of the calprotectin subunits S100A8 and S100A9 in VAT. (A, B) Gene expression levels of S100A8 and S100A9 in VAT of LN, obese NG and obese T2D volunteers. Bars represent the mean ± SEM of the ratio between the gene expression to 18SrRNA. The expression level in LN subjects was assumed to be 1 (LN: n = 9; NG: n = 10; T2D: n = 10). Differences between groups were analyzed by one-way ANOVA followed by Tukey tests. *P < 0.05, **P < 0.01, versus LN, and †P < 0.05 versus NG. (C, D) Comparison of S100A8 and S100A9 gene expression in adipocytes and SVFCs isolated from VAT of obese patients. Bars represent the mean ± SEM of the ratio between the gene expression to 18S rRNA. The expression level in adipocytes was assumed to be 1 (adipocytes: n = 9; SVFCs: n = 11). Statistical differences were assessed by two-tailed unpaired Student ttests. ***P < 0.001 versus adipocytes.