Figure 2
From: Cannabinoid Receptor Type I Modulates Alcohol-Induced Liver Fibrosis
The effect of acetaldehyde, H2O2, hypoxia and endo- and exocannabinoids on CB1 and fibrosis-related mRNA in HSC. (A) CB1 and (B) CB2 mRNA after the treatment with 50–200 µmol/L of acetaldehyde (AA) (6 and 24 h) (human HSC). (C) CB1 mRNA after the treatment with 1–50 µmol/L H2O2 (24 h), (D) CB1 mRNA under hypoxic conditions (1% O2) (24 h), (E) CB1 mRNA after 24 h treatment with 2-AG (20 µmol/L) or THC (1 µmol/L) alone, or in combination with AA (200 µmol/L), and (F) αSMA mRNA after 24 h treatment with 2-AG (20 µmol/L) or THC (1 µmol/L) alone, or in combination with AA (200 µmol/L) (rat HSC); measured by TaqMan PCR and normalized to GAPDH (mean ± SD; *P < 0.05, **P < 0.005).