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Figure 5 | Molecular Medicine

Figure 5

From: Homeobox D10 Gene, a Candidate Tumor Suppressor, Is Downregulated through Promoter Hypermethylation and Associated with Gastric Carcinogenesis

Figure 5

Gene expression profile changes by ectopic expression of HoxD10. (A) The gene expression profiles in HoxD10 or control vector stable transfectants was visualized in MKN28 cell lines. Representative genes with over a two-fold change are indicated on the right side of this heatmap image. (B) The expression of nine genes was measured by qPCR and calculated using the value of 2−ΔΔCT. Relative fold changes of HoxD10 transfectants were compared with empty vector values normalized to 1. □, Result of cDNA microarray; ■, qRT-PCR data in MKN28 cells. (C) Western blot analysis of IGFBP3 and caspases. The expression of IGFBP3, caspase 3 (pro and cleaved) and caspase 8 (pro and cleaved) were detected after being transected with HoxD10or empty vector in gastric cancer cell lines (AGS and MKN28). Band densities were normalized to β-actin. Relative fold change was expressed when compared with that of empty vector.

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Molecular Medicine

ISSN: 1528-3658