Figure 7

DNA promoter of HoxD10is hypermethylated in gastric cancer. (A) HoxD10 expression was examined in gastric cancer cells (AGS, MKN28, MKN45 and NCI-N87) after treatment with 5-Aza for 3 d (d3) by RT-PCR (left diagram) and Western blot (right diagram). (B) HoxD10expression was examined in gastric cancer cells (AGS, MKN28 and MKN45) after withdrawn of 5-Aza for another 2 d (d5). Aza: 5-aza-2′-deoxycytidine. (C) The methylation status of HoxD10promoter is detected by methylation-specific PCR (MSP) in the above gastric cancer cell lines. (D) The methylation status of HoxD10promoter in primary gastric cancer, intestinal metaplasia and normal stomach tissues was detected by MSP. Representative images are shown. T: Gastric tumor tissues; A: Adjacent nontumor tissues; IM: Intestinal metaplasia with atrophic gastritis tissues; N: Normal stomach tissue; M: Methylated; U: Unmethylated. (E) Survival curves were plotted based on Kaplan-Meier survival analysis. Methylation status of HoxD10promoter was used as the variate to separate two lines (n = 58, P = 0.028, log-rank test). , Unmethylated; , methylated.