Figure 2

SHIP1 inhibition affects apoptosis induction differently in MM cell lines. (A) MM cells were treated with increasing concentrations of 3AC for 48 h and Annexin V-positive cells were determined by fluorescence-activated cell sorter (FACS) analysis. Results represent the mean increase in apoptotic cells in two independent experiments. ▤, U266; ▦, RPMI8226; ▩, OPM2. (B) MM cells were treated for 48 h with increasing concentrations of 3AC. After cell lysis, proteins were separated by SDS-PAGE and blots were probed with antibodies against hallmark proteins for apoptosis or autophagy (upper panel: cleaved caspase 3, cleaved caspase 9, LC3B-II; lower panel: cleaved PARP). Equal loading was confirmed by reprobing blots with antibodies against actin. conc, concentration.