Figure 6

Pan-SHIP1/2 inhibitors reduce viable cell numbers of SHIP2-expressing MM cells. RPMI8226 (A), U266 (B) and OPM2 (C) cells were treated with increasing concentration of pan-SHIP inhibitors for 36 h, and cell viability was determined with a cell counting kit. The mean of three independent experiments is shown. Asterisks indicate the concentration (conc) at which cell killing became significant for each compound. Double asterisks indicate at which concentrations 2PIQ showed significantly less cell killing than 1PIE or 6PTE. —●—, 1PIE; —■—, 6PTQ; —▲—2PIQ. (D) Untreated MM (RPMI8226, U266 and OPM2) cells and breast cancer cells (MCF-7 and MDA-MB-231) were lysed, and proteins were separated by SDS-PAGE, after which the blots were probed with antibodies against SHIP1, SHIP2 and PTEN. Equal loading was confirmed by reprobing the blots with antibodies against actin. conc, concentration.