Figure 8
Pan-SHIP inhibitors reduce viable cell numbers of SHIP2-expressing breast cancer cells. MDA-MB-231 (A) and MCF-7 (B) cells were treated with indicated concentrations of pan-SHIP inhibitors for 2 h. Cells were washed twice, and fresh medium was added. Viable cell number was determined with a cell counting kit after 24 h, and results represent the mean of at least two independent experiments. —●—, 3AC; —■—, 1PIE; —▲—, 6PTQ; —▼—, 2PIQ. (C–E) After treatment of MCF-7 cells with 10 µmol/L 1PIE, 2PIQ or 6PTQ for 2 h, cells were subsequently cultured for 36 h in the absence or presence of the indicated concentrations of PtsIns(3,4)P2 or PtdIns(3,5)P2, after which cell viability was determined with a cell counting kit. The mean of three independent experiments is shown. —●—, PI(3,4)P2; —■—, PI(3,5)P2. (F) MCF-7 cells were treated with 10 µmol/L 3AC for 2 h, after which the medium was replaced and cells were cultured in the absence or presence of indicated concentrations of PtsIns(3,4)P2 or PtdIns(3,5)P2 for 36 h. Cell viability was determined with a cell counting kit. —●—, PI(3,4)P2; —■—, PI(3,5)P2.