Figure 4

SAA upregulates MCP-1 secretion in atherosclerotic plaque of chow-fed mice. (A, B) Immunohistochemistry staining and quantification of MCP-1 (brown, bar = 100 µm; n = 10 for both groups). (C) Real-time PCR analysis of mRNA expression of MCP-1. Relative expression was normalized to that of reference genes β-actin TBP and GAPDH. Geometric mean of reference gene expressions was used to confirm the robustness of experimental data. Data are mean ± SD. *P < 0.01 compared with the lenti-null group (n = 12 for both groups).