Figure 2
Iloprost enhanced IL-10 expression and suppressed poly I:C-induced TNF-α expression in mDCs via the IP and EP receptors in human mDCs. Isolated human mDCs were pretreated with IP receptor antagonist CAY 10449 or EP receptor antagonists 1 h before treatment of iloprost. (A) CAY 10449 reversed iloprost-enhanced IL-10 expression. (B) The EP1 receptor antagonist SC19220 (10−5 mol/L), but not EP2 or EP4 receptor antagonists, also reversed iloprost-enhanced IL-10 expression. CAY 10449 (C) and EP1, EP2 and EP4 receptor antagonists (D) all significantly reversed iloprost-suppressed poly I:C-induced TNF-α expression. Results presented are the mean ± SD of six independent experiments using mDCs from six subjects. *P < 0.05 compared with iloprost-treated cells (A, B) or iloprost plus poly I:C-treated cells (C, D).