Figure 3
Treprostinil enhanced IL-10 expression via the EP4 receptor and suppressed poly I:C-induced TNF-α expression via the IP receptor in human mDCs. Isolated human mDCs were pretreated with IP receptor antagonist CAY 10449 or EP receptor antagonists 1 h before treatment of treprostinil. (A) CAY 10449 was unable to reverse treprostinil-enhanced IL-10 expression. (B) EP4 receptor antagonist GW627368X (10−5 mol/L), but not EP1 or EP2 receptor antagonists, reversed treprostinil-enhanced IL-10 expression. (C) The IP receptor antagonist CAY 10449, but not the EP1, EP2 or EP4 receptor antagonist, reversed treprostinil-suppressed poly I:C-induced TNF-α expression in mDCs. Result represented the mean ± SD of six independent experiments using the mDCs of six subjects. *P < 0.05 compared with treprostinil-treated cells (B) or treprostinil plus poly I:C-treated cells (C).