Figure 4
The modulatory effects of PGI2 analogs on IL-10 and TNF-α expression is independent of PPARs in human mDCs. To investigate the involvement of PPARs in the modulatory effect of PGI2 analogs on IL-10 and TNF-α expression in human mDCs, the cells were pretreated with PPAR-α antagonist (GW 6741) and PPAR-γ antagonists (GW9662) 1 h before the treatment of iloprost or treprostinil. Neither PPAR-α antagonist nor PPAR-γ antagonist changed the enhancing effect of iloprost (A) or treprostinil (B) on IL-10 expression, and the suppressive effect of iloprost (C) or treprostinil (D) on poly I:C-induced TNF-α expression. Results represented are the mean ± SD of three independent experiments using mDCs from three subjects.