Figure 8
Iloprost-treated mDCs suppressed IL-13, IFN-γ and IL-10 production by CD4+ T cells. Autologous CD4+ T cells were isolated and then cocultured with vehicle-treated or iloprost-treated mDCs (105 mDCs/106 T cells) for 5 d in the presence of anti-CD3 and anti-CD28 antibodies with or without the addition of IL-10 neutralizing antibody. Supernatants were collected for IL-13, IFN-γ and IL-10 measurement. Iloprost-treated mDCs significantly suppressed IL-13 (A), IFN-γ (B) and IL-10 production (C) by CD4+ T cells. (D) The addition of IL-10 neutralizing antibody did not change the effect of iloprost-treated mDCs on IL-13 and IFN-γ production by T cells. Results represent the mean ± SD of six independent experiments using mDCs from six subjects in the experiments without the addition of IL-10 neutralizing antibody and of three independent experiments using mDCs from three subjects in the experiments with the addition of IL-10 neutralizing antibody. *P ≤ 0.05 compared with the group of vesicle-treated mDC/T cell coculture. §P ≤ 0.05 compared with the group of iloprost-treated mDC/T-cell coculture without the addition of IL-10 neutralizing antibody. (D) □, Control; ■, iloprost (10−7 mol/L); ■, iloprost (10−7 mol/L) + anti-IL-10.