Figure 5

Effect of A1AT on the activity of different classes of caspases. Cell-free kinetic enzymatic activity was measured as follows for different classes of caspases. (A) Executioner caspases: recombinant active caspases -3 (●), -6 (V) or -7 (■) were incubated with purified human A1AT (0-500 µg/mL). (B) Initiator caspases (recombinant active caspases -8, -9, -10, -2), executioner caspases (-3, -7, -6) or inflammatory caspases (-1, -4, -5) were incubated with purified human A1AT obtained from pooled plasma (500 µg/mL; n = 3). (C) Executioner caspase-6 incubated with purified A1AT (■) or recombinant RCL-null A1AT (■) (0-500 µg/mL; n = 3; *P < 0.05 versus caspase-6 activity incubated with vehicle and versus RCL-null A1AT).